FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42222624
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5428111.2855927665698845No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA864030.2046367369304191No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA732580.17350413844482995No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGC687760.16288897629858343No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA526620.12472460262062349No Hit
GTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTC511990.12125963559252026No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT505420.11970359776786967No Hit
CCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCG491450.11639494504178613No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG465000.1101305309684211No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA458000.10847265200760617No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC451320.1068905617992856No Hit
CCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGG432920.10253270853085776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT38700.025.5229556
CCGCGTT41750.023.9281737
GTCGGAT41250.021.2236881
AGACCGC47450.021.148484
GCGTTCT47100.021.019219
ACGTTCG88850.020.4105646
CAATACG47250.019.90443431
CGTTCGA90800.019.8485957
GAACGAT46800.019.56714645
CAGACGT94300.019.5650353
AGACGTT92700.019.4416584
GCAGACG94800.019.201772
AATACGG48800.019.13370132
TACGGAG47700.018.91453734
GACGTTC96550.018.8759865
GGGTCGT98450.018.46666716
ATACGGA51100.018.05226333
TCGGATT54500.018.0420552
CGCGTTC56650.017.8729698
TGGGTCG106600.017.20254515
Produced by FastQC (version 0.11.5)