FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4983
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA771.545253863134658No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACC100.2006823198876179No Hit
GTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCG80.16054585591009432No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.14047762392133253No Hit
GTTTGCCTCCCTTTGTGCCATGATTGTAAGTTTCCTGGGGTCTCCTTAGCC70.14047762392133253No Hit
CTCCATTCCATTCCACTCCACTCCACTCCACTACAATCCACTCCATTCCTC70.14047762392133253No Hit
TTTAAGATGTATTTTTGAGTTGGGTTAATCTTATGTTTATATGGCTATTTA70.14047762392133253No Hit
TGAACCCCCAGGTGGGCCAGACGTTCGTGGTGGCCGATGGGGCGGCCAACA70.14047762392133253No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC70.14047762392133253No Hit
GCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTC70.14047762392133253No Hit
CGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGC60.12040939193257075No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12040939193257075No Hit
GTTACAAAGTAAAAACGAACAGTACATTCCTTCAACTGACAAGAAGCTATA60.12040939193257075No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12040939193257075No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC60.12040939193257075No Hit
CAAAACCTCAGTCTTCTCTATAACTCATACATAGCACTAGCTTCTAATATT60.12040939193257075No Hit
AAGAAAGAAAGAAGAAAGAAAGAAGGAAGGAAGGAAGGAAAGAAAGAAAGA60.12040939193257075No Hit
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGG60.12040939193257075No Hit
CCTTGCAGGAGGCTGCTGCATGCAAACCCAAATTCTCTCCATTCTTGGAAT60.12040939193257075No Hit
CTGCTGTCGATATCCTGAAGACTGGTTTCTATTGTTCCCTGAGAACTGGAA50.10034115994380895No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC50.10034115994380895TruSeq Adapter, Index 7 (100% over 51bp)
CTCTTCTTCAATCTGCAGCTGCCTAGGCATTTAACCTGAGGCTTATGAATA50.10034115994380895No Hit
ATTGAGTCCGTTTTATTCCAGTCCACTGCATTCTGGTCCATTCCATTTGAT50.10034115994380895No Hit
CCCGTCCATACATAGCTACCCAGCTATGCCGTTGGCACGACAACTGGTACA50.10034115994380895No Hit
AATTATTTCTCAGGTGCAAACCCAGTTTCCCACTTCTGTAACCTTATCACA50.10034115994380895No Hit
TTTTACTTTATCATATGCATCATATAAACACTGTTATGAAGGTGTTTTTTC50.10034115994380895No Hit
GCCCAAATCCCCACATGCCAAAAGCTCCTACCTGACCAGCCCTGTCCACCC50.10034115994380895No Hit
GGAGAATCGCCTGAACCCAGGAATTCAAAACTAGCCTAGGCAACAGAGTGA50.10034115994380895No Hit
CCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAA50.10034115994380895No Hit
TGAATCCCTTGTTTTCTGGGGATTGTGGCTTAAGTTCCAATGTTGAAGACA50.10034115994380895No Hit
TGCAAATATCCTGCATCTGCTTAAATTTTCAAACATTAATATTAACATCTC50.10034115994380895No Hit
GGAAGCACTTTTCTGTGTATGTATGATGTTTCACATTAAACATTTTCTTTT50.10034115994380895No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTTCCGTATCTCGTA50.10034115994380895TruSeq Adapter, Index 7 (97% over 39bp)
CCTGTGCATGCGGTTTGCACATCATTGTCCTTGTCATGGCTTCCATGTGGG50.10034115994380895No Hit
CTCAATACTAATGGAAGTAATCTGCTTAACAATCTCAGAAGGACTGTGCAA50.10034115994380895No Hit
GCTGCAGTTTAGATTGTAATTTCTTTCTTGGGTAACTGGAGATAGTTTACT50.10034115994380895No Hit
GGAAGGGAGCTGGTGTGTGTAGAGATCACACAGCAAAAGAGAAAGCAAGAG50.10034115994380895No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)