FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11938111
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA128470.1076133401674687No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG121470.10174976593868158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC12900.025.11553413
CCCGTAT4150.023.867211
CGTCTGA13750.023.23548116
GATCGGA14500.022.6663671
ATCGGAA14650.022.4249842
TCGGAAG16200.020.4182853
ACGTCTG16300.019.87654315
GAACGTG27500.019.30948826
GCACACG17700.019.19434511
GGAACGT27400.019.13360625
CACACGT18250.018.7391712
TGCGCCA19100.018.72988745
CCTTGCG19950.018.72218925
CGGAAGA19200.018.633824
CGGAGCG10550.018.55379739
CTTGCGG19900.018.31696126
TGTTTCG8050.018.16754333
CACGTCT17850.018.15056814
GAGCGCT10550.018.12727241
CGCGTTG1253.8208418E-718.0088941
Produced by FastQC (version 0.11.5)