FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23396607
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG42100.022.81801442
ACCGCGT10800.022.7056476
CGCGTAT2100.020.3643951
CCGCGTT12150.020.182977
ACGTTCG8300.018.973666
CCTTGCG51700.018.84484125
AGACCGC13650.018.7892274
TTGCGCC51950.018.62151144
TGCGGTA54750.017.91683628
CTTGCGG54650.017.90994526
CGTTCGA9050.017.8985867
GCGGTAC53450.017.88938929
TTGCGGT54850.017.51646227
TGCGCCA56550.017.34579845
GTCGGAT7700.016.661781
CCCTACG60650.016.58393924
GTCGGTA4650.016.4574741
CCCGTCT61400.016.349471
GTCGATT8850.016.2769831
CGCGTCT6950.016.1928181
Produced by FastQC (version 0.11.5)