FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23128293
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC580360.25093075394712444No Hit
GTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCT536610.2320145286986809No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA527880.22823993106624857No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGA510090.22054805341665293No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG508900.22003353208989526No Hit
GGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT407180.17605276792368552No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA359250.15532923246864783No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT353600.15288633709370597No Hit
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCA343370.14846318316704135No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACC316880.13700967901089803No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAG279120.12068335523075567No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGG261290.11297418274664715No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC257150.11118416737456585No Hit
GCCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGA252790.10929903041266383No Hit
GGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTA244250.10560658324416765No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGG235330.10174983514779927No Hit
GTTCAGATATGTTAAAGCCACTTTCGTAGTTTATTTTGTGTCAACTGGAGT231580.1001284444122184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG62050.027.37599242
TGCGCCA64550.026.45566645
GTCGTCG13400.026.0252218
ACGTTCG13900.025.898226
CGTCTGA29550.025.58270816
TTGCGCC67400.025.10282744
CGCCCCT30850.025.08811216
CTTGCGG69550.024.78089726
CCTTGCG70350.024.5630625
ACACGTC31300.024.15283613
TCGTCGC14950.023.62819
TTGCGGT71650.023.58354827
GCGGTAC72400.023.24550829
TGCGGTA72850.023.2259128
ACGTCTG33300.023.03959815
ACGCCGG129400.022.79584322
TACGCCG132300.022.72089421
TCGCCGC15650.022.71590222
CGATTAG37350.022.351144
GGGTGAT153700.022.3368421
Produced by FastQC (version 0.11.5)