FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9559954
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG201070.21032527980783172No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG173760.18175819674446134No Hit
GGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGA160710.1681075034461463No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGC156970.1641953507307671No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC145450.152145083543289No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA136350.14262620928929157No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT127420.1332851601587204No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGC127110.13296089081600182No Hit
CTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCT127090.1329399702132458No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTG116620.12198803467045971No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG108070.1130444769922533No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT99880.10447749016365561No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGC99370.10394401479337662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCT12800.025.48642341
AGCGCTG14100.022.9776542
CGCTGAG14850.022.57482144
ATACCGC15900.020.94285627
GCGCTGA15700.020.77928443
CGGAGCG17200.020.01307739
CTGGCGG18100.018.6450535
ACCGCGT3000.017.9991596
CCGCGTT3000.017.2515417
TGGCGAC6800.017.2057082
GGAACGT58650.017.14852325
TGCGCCA22250.017.08989345
AACGTGA61500.016.5361627
ACGTGAG61350.016.46648228
GAACGTG61250.016.4573226
GGATACC20750.016.26523625
CCGCCTG20700.016.08738119
GGAGCGC20700.015.97709740
GCCGCCT20750.015.93968118
GTGGCGA8050.015.9396031
Produced by FastQC (version 0.11.5)