FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12282487
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA24250.029.22597516
TCGTATG15900.029.15035445
ACACGTC24800.028.21538413
ACGTCTG25200.028.12420315
TCTCGTA18850.025.90112143
ATCTCGT18650.025.334442
TATTGCG28950.025.18063242
GCACACG30650.024.37164711
CACGTCT29250.024.1531614
AATCTCG18300.023.72883641
CACACGT31650.023.5305212
AGTCACG13100.023.18730728
TCGGAAT17250.023.086337
TGTTTCG9400.022.49945333
ATCGGAA34750.022.2748342
GATCGGA35000.021.9959261
TTTCGGA18450.021.82873535
GCGGTAC33000.021.5449329
CGGAAGA36650.021.4267854
CCTTGCG33750.021.26692825
Produced by FastQC (version 0.11.5)