FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46754959
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG707210.15125882155088619No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGC658510.1408428141280158No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA569360.1217753179935416No Hit
GGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGA562810.12037439707732393No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG527970.11292278108938135No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGC503670.10772547143074172No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT488560.10449372867592506No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGC485400.10381786453924598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCT67350.027.22580141
CGGAGCG75000.025.28864739
AGCGCTG74900.024.60156842
CGCTGAG79000.023.7242544
GCGCTGA79450.023.4758543
CTGGCGG92000.021.05598335
GGAGCGC92250.020.16965340
GCGGAGC100800.019.083838
CCTGGCG110650.017.58834334
ATACCGC40600.017.23625427
TACGTTC87000.016.16476227
TGGCGGA115950.015.95002236
CCTACGT89000.015.92800425
GTCGTCG33000.015.81789318
TGGCGAC24650.015.7001662
GGAACGT209500.015.57380925
CTACGTT91400.015.46046126
ACCGCGT7000.015.4277796
CGTTCGA36200.015.3521437
TTCGTGA61150.015.30634544
Produced by FastQC (version 0.11.5)