FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6971
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.8033280734471382No Hit
GTGCTCCTATTAGAATCTAATGCCACAGCTGATCTGACAGGAGGCGGAGCA120.17214173002438674No Hit
GAAAAAAATCAAGAAGTACTTAACTTTTGCTGAGAAACTGAATAAAACATA110.15779658585568784No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG90.12910629751829006TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC70.10041600918089227TruSeq Adapter, Index 12 (100% over 51bp)
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA70.10041600918089227No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)