FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49797869
Sequences flagged as poor quality0
Sequence length126
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA12850.098.508186
TCCGATC13350.094.3693548
GCTCTTC15400.081.0280763
CGCTCTT19150.065.787512
TCTTCCG19550.064.7483445
TTCCGAT22100.059.449037
CTCTTCC23300.053.2975654
CCGATCT28500.043.391449
ACGATCC4400.032.72331120
ACGCTCT38850.032.4500121
TTTCCCC83700.032.253743
ACTGAAC5500.030.541752120
ACGAACC2950.030.504778120
TTCCCCC82700.029.451914
CGATCTA7800.026.55556510-11
TTTTCCC119300.023.232422
ATGCCGC15050.023.120123120
CGATCTG7100.022.83160610-11
AAACGAC94950.022.556507120
TTACCCC9200.022.1709374
Produced by FastQC (version 0.11.5)