FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49797869
Sequences flagged as poor quality0
Sequence length126
%GC29

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTG34750.057.494785120
TCGCCGG9300.050.321167120
TCTCGGG16400.048.65717120
GTCGTGG25300.047.429493120
GCGTCGG105050.045.691242120
ATGGTCG1750.044.57017120
TACTAGG558.188661E-443.63514120
AAAGAGG74700.042.810043120
AGAGTGG6700.040.297375120
ATGATGG600.001256454439.998875120
GGAAAGG63700.039.559326120
GTAGGGG30950.038.96498120
GAGTGTG4500.038.66558120
TCGAGTG801.1408045E-437.498947120
GAGTGTT1452.4629117E-937.24033120
AGTGGTC1303.9108272E-836.92204120
GCATCGG1303.9108272E-836.92204120
CGGTGGG13550.034.537773120
GGTATCA1051.4025261E-534.284748120
AGAGCGG74600.033.698784120
Produced by FastQC (version 0.11.5)