FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14025696
Sequences flagged as poor quality0
Sequence length126
%GC29

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACAC9300.053.54947120
TTCCCCA1800.053.334984
ATGCCGC2250.053.33441120
TTTCCCC35050.048.788953
ATTTCCC2300.047.026711
TACATCC1850.045.406322120
TTCCCCC33350.044.97894
TTTTCCC41350.043.096552
CCTATCC1002.0865627E-741.9992038
CTATCCC1151.326589E-841.7383359
TGGCCAA300.001255783340.00316222-23
CCCTATC957.0594615E-637.895917
CTCAAGC401.1403482E-437.50142724-25
CTTAACC851.6291132E-435.29483120
GCTATCC350.002677583434.2866526-27
CCAGTCC8750.032.91495120
CAGGCTC551.9275445E-532.72816526-27
CTGGCAG551.9275445E-532.72816526-27
CTAGCAG551.9275445E-532.72816526-27
TCCCCCT8900.032.360555
Produced by FastQC (version 0.11.5)