FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14025696
Sequences flagged as poor quality0
Sequence length126
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG3600.054.96464120
TCGCCGG2150.050.200264120
GCGTCGG26250.045.456474120
GTCGTGG6350.043.436626120
GGAAAGG14400.043.305477120
GAGTGTG1255.7843863E-1043.17223120
AAAGAGG18850.042.625103120
TCGTGTG11550.041.01258120
GGTGGTG2100.039.97429120
GTGGTCC650.001867451536.89934120
TTAGATG1001.0082151E-535.97686120
CGGTAGT851.635478E-435.271427120
TTCACGG350.002675821534.29125698-99
GTAGGGG7650.031.352383120
TAGATCC4900.030.592567120
GTGTTGT400.005165098229.981197118-119
CGGAAGG15850.029.507833120
GGGTGTG1453.8335456E-628.946898120
AGAGCGC27350.028.939337120
TGAGAAA1254.6357687E-528.781485120
Produced by FastQC (version 0.11.5)