FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19991119
Sequences flagged as poor quality0
Sequence length126
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGACC152.6132065E-4120.007385120
ACGCTCT1100.087.28051
CGCTCTT1250.076.800882
CTTCCGA1500.067.997726
TCCGATC1550.065.80318
GCTCTTC1600.063.7416463
CCGATCT1650.061.8150259
ACCATAC701.2567398E-860.00369120
TCTTCCG1950.052.299795
ATCCGCG350.007541701751.433151
CAACAGC350.007542522651.431736120
CTACACC505.1175355E-448.002956120
TTCCGAT2500.045.5976687
CTCTTCC2350.043.398244
CGACAAA705.190521E-542.85978120
TCTGTCC300.001255905440.00241118-119
AGTCACC4700.038.30023120
TATGCCC4700.035.74688120
CATCTAC700.002677116334.287823120
ACTACCA700.002677116334.287823120
Produced by FastQC (version 0.11.5)