FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19991119
Sequences flagged as poor quality0
Sequence length126
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA516130.25817964467121624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTCG1507.8216544E-1139.998474120
GTGGTCC902.2801022E-433.33206120
TCTCGGG7450.032.213535120
TCAGTTG400.00514539430.004677118-119
TCGCCGG5400.029.998856120
GTAGATT6850.025.400492120
AGTGTAA14900.024.96549120
TGTGGTC2153.1880209E-622.32473120
CGCTGGT1354.1589374E-822.225685118-119
GCGGTGG3451.1605152E-920.868769120
GTCGAGG1450.003687266520.688866120
TGCCGTA2055.5741337E-520.487024120
TGACGAG750.00447869920.005695114-115
CGGTGGG7100.019.435879120
TAGGTAG1550.00542388719.354101120
GCGTCGG46650.017.877132120
GTCGTGG13700.017.51758120
GGTTGCC1201.8649262E-417.503363116-117
AAAGAGG51950.017.439182120
GCTGGTC1403.1125135E-517.145527118-119
Produced by FastQC (version 0.11.5)