FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15234048
Sequences flagged as poor quality0
Sequence length126
%GC28

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT3900.0109.230049
CTTCCGA3950.0107.842776
TCCGATC4050.0106.661418
ACGCTCT4100.0101.091061
GCTCTTC4650.087.737613
TCTTCCG5050.084.352275
CGCTCTT5100.081.172462
TTCCGAT6200.071.609377
CTCTTCC6300.064.758714
AAGCACC951.3944918E-744.209946120
ATGCCGC2700.042.22166120
TAACGCC401.1404082E-437.50110620-21
TCAGCTA501.0036963E-536.00064530-31
GCCTATG608.7083936E-735.0006330-31
GTCTTCC1051.402319E-534.285263120
GCCGTCC1051.402319E-534.285263120
TTTCCCC23050.033.837813
AAACCTG452.2786984E-433.3356328-29
CAGTGGC452.2792254E-433.33431620-21
GAGACCC750.003755192531.999577120
Produced by FastQC (version 0.11.5)