FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14267943
Sequences flagged as poor quality0
Sequence length126
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGAG208.2077115E-489.99572120
TCGCCGG3150.062.854156120
TTGTAGA401.7012241E-459.997147120
GTCGTGG7900.057.718773120
CGGTGGG3550.054.081932120
AGAGTGG1750.051.42613120
GCGTCGG30450.048.864674120
AAAGAGG20700.048.693336120
GAGTGTG1251.0913936E-1147.99772120
AGATTGT402.16851E-644.996437116-117
GGAAAGG15600.044.613262120
AGTGGTC558.1892486E-443.63429120
TTCGGTG558.1892486E-443.63429120
GGTGGTG1551.8189894E-1242.57862120
AGAGTAA853.2899807E-642.35093120
TCGTGTG8100.041.479507120
GTAGGGG8950.040.89191120
GGTAAGG757.80132E-539.998096120
GACGTGG600.001256544439.998096120
TCTCGGG4650.039.998096120
Produced by FastQC (version 0.11.5)