FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13569992
Sequences flagged as poor quality0
Sequence length126
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA807.2759576E-1267.493186
CCGATCT803.5741323E-852.5576329
CTCATGC255.1176746E-448.00275424-25
TCCGATC1108.965799E-943.6319548
TTTCCCC30400.043.4166643
ATTTCCC2650.043.0654561
TTTTCCC34400.040.4610252
TTCCCCA1707.2759576E-1238.8196074
ACGCTCT1104.3692125E-738.2231671
CAGATCC401.14021735E-437.5021526-27
CCTAGAG501.0039155E-535.99947730-31
CTATCTG1100.035.4565826-27
TTCCCCT8400.034.9964644
TTCCCCC28250.034.615974
ACGATCC350.002677187634.2876824-25
AAACCTG452.2790428E-433.3347528-29
CCAGTTG452.2798074E-433.33284830-31
AGCCTGA551.9273395E-532.7286728-29
AGACTGC400.005147842730.00172224-25
AGATCCA802.421184E-730.00172226-27
Produced by FastQC (version 0.11.5)