FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13569992
Sequences flagged as poor quality0
Sequence length126
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG4450.068.604744120
TAGATGA150.004137286559.861004118-119
TTAGTGC150.00413758859.8599116-117
CGGTTAG453.0788165E-453.20978120
GCATTAG350.00761379851.30943120
TCTCGGG7400.050.962746120
GTCGTGG10700.048.672028120
GAATGTA2400.047.86953100-101
GTAGGGG14100.045.850983120
GAGTGTG2100.045.608387120
ATGTAGA2900.044.9697102-103
AATGTAG2900.043.89899102-103
TGGTCGT354.73158E-543.53753396-97
GCGTCGG42650.043.369404120
ATTGTAG801.8189894E-1242.694454102-103
AGAATGT2550.042.618347100-101
TCGTGTG15300.042.254826120
AAAGAGG30550.041.73615120
GGAAAGG22250.041.700924120
CGGTGAT1050.041.4006898-99
Produced by FastQC (version 0.11.5)