FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21663284
Sequences flagged as poor quality0
Sequence length126
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTG1150.057.255844120
GTCGTGG7850.057.189537120
TCGCCGG1800.056.532917120
GTAGGGG8200.055.478504120
GCGTCGG32100.053.33177120
TCGTGTG9700.052.453224120
TCTCGGG2950.050.727448120
CGGTGGG2600.043.742664120
AAAGAGG20100.042.58582120
ACGTCGG853.3428605E-642.25298120
ATGGTCG601.41626515E-841.43058102-103
GGAAAGG15950.040.90636120
CTAGCTG300.001272417439.8962982-83
TCTAGGG3100.038.0894102-103
CTAGGGA3100.038.0894102-103
GAATATG401.1566671E-437.41106116-117
TCGTGGG650.001883311536.83593120
GTAGATT650.001883311536.83593120
GTCTAGG3250.036.331432100-101
CGGTGCT350.002253993235.5119298-99
Produced by FastQC (version 0.11.5)