FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38012842
Sequences flagged as poor quality0
Sequence length126
%GC29

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA3450.090.430316
ACGCTCT4350.077.324221
CCGATCT3950.075.949219
TCCGATC4000.074.996298
GCTCTTC6200.051.2877853
TATGCCC2700.048.887695120
TCTTCCG6450.048.3696985
ATGCCGC5350.038.129906120
CGCTCTT9100.036.921252
TTTCCCC78050.034.745243
TTCCCCA4150.031.8056564
TTCCCCC73100.030.3678554
GTACACC800.00514992829.999268120
CTCTTCC10700.029.1574364
TTCCGAT11000.028.907667
TTTTCCC100850.028.7938182
TGCCCTG656.0279737E-527.69368728-29
TCAGCCT557.38344E-427.2742326-27
GACAATC1005.646143E-827.00157524-25
CTGTCAG906.783157E-726.66813726-27
Produced by FastQC (version 0.11.5)