FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16184347
Sequences flagged as poor quality0
Sequence length126
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC250.001990720971.994948
CGCTCTT401.69872E-460.0150682
ACCGAGC150.004097248460.0082158-59
CCGATCT300.00410057259.9957859
ACGCTCT505.110076E-448.01711
GCTCTTC650.001861283136.924253
GTCACTT6850.032.406483120
CTGCCGT400.00515106929.997892110-111
ATGCCGC1254.6177433E-528.797976120
AACCATT900.00915465726.664793120
AACGCCA808.203926E-626.248602106-107
CTGCTTT3450.026.085123120
TGCCGTT1858.624156E-725.944122120
GCCAGTC709.988449E-525.712479110-111
AGCTTAA13600.025.145292120
TCTACTT2401.1943484E-824.998243120
CCAGTCC5100.024.704147120
TCACTAA6650.024.359192120
CGCCAGT751.5956159E-423.998316108-109
AATCACA2052.1084634E-623.412989120
Produced by FastQC (version 0.11.5)