FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16184347
Sequences flagged as poor quality0
Sequence length126
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA268210.16572185457961325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTC1150.033.923214112-113
AGTGTAA10450.032.162407120
TCCGTGG1058.367351E-1131.437899110-111
GTGGTCC1352.180699E-631.120106120
GATGTCG1450.031.042496108-109
TGTCGGT1500.030.008905110-111
TCGGTGC1001.7043931E-930.004406106-107
ATGTCGG1550.029.040876110-111
GGTCTCG1052.8667273E-928.579908110-111
TAGGTCT1052.8685463E-928.578804108-109
GTAGATT5350.028.045488120
GTCTCGG1104.7093636E-927.280907112-113
CGGTGCT1104.7184585E-927.276733106-107
TCTCGGG4000.027.007803120
GTGTACG1453.6379788E-1226.900503106-107
CCGTGGT1355.2750693E-1126.674582110-111
AGTCTAG1800.026.673552108-109
GAGTGCA1800.026.670584106-107
CGTCCTG2050.026.348265108-109
GTAGATG2050.026.345331106-107
Produced by FastQC (version 0.11.5)