FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32279543
Sequences flagged as poor quality0
Sequence length126
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN909610.28179147393753373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA465680.1442647437728595No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTAG602.0744186E-550.058228120
GCACAGG951.3817225E-744.26201120
TCTCGGG15300.034.54999120
GGACTGG700.002662710434.325638120
CAGCAGC1004.2001109E-430.034935120
CGTCAGC800.005120082330.034935120
CAAGAGG2651.8189894E-1229.468237120
TCAAAGG1055.5886596E-428.6047120
TGGAGGC850.00688524228.268173120
TGAGACG557.409302E-427.257908118-119
TCGCCGG6900.026.987911120
ATCAGGG900.00909987326.69772120
GATCAGG900.00909987326.69772120
GTAGATT11750.026.584112120
ATCATGG701.0009928E-425.70441114-115
GGTATCT1200.001218976925.029114120
CGGTGGG11850.023.31826120
GCATGGG650.001962055523.064383118-119
TGGCAGC802.474797E-422.492952116-117
AGGTATG802.4786568E-422.487772118-119
Produced by FastQC (version 0.11.5)