FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17482953
Sequences flagged as poor quality0
Sequence length126
%GC29

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179100.10244264798973035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT1150.0119.992539
TCCGATC1200.0114.992848
ACGCTCT1550.0104.554781
CTTCCGA1450.099.308996
CGCTCTT1750.092.594262
TCTTCCG1800.080.002575
GCTCTTC1900.078.9499053
TACCACT300.004100401459.996437120
TTCCGAT2450.058.7730267
CTCTTCC2700.053.335054
CGATCTA1150.041.7365310-11
CTACATT600.001256625239.997623120
CCCGTAT650.001862120136.920887120
CAGTACA1051.4027737E-534.28368120
CCAGTCC4650.032.25615120
GAGTCTC750.003748173432.0118331
CAACTAA750.00375605631.9981120
TTCCGCG800.00513993730.0110931
CACCGTT3300.029.08918120
CGCCAGT953.435889E-828.419325108-109
Produced by FastQC (version 0.11.5)