FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8999266
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG5300.061.07975120
ATGATGC150.004112142659.953136116-117
TCTCGGG7600.047.327877120
GTCGTGG6700.034.895477120
CGGTCCT350.002672078834.3009880-81
CGGTGGG6150.033.142338120
GCGTCGG26300.033.051533120
GAGTGTG2400.032.47218120
GTGAGCG750.003770644331.972607120
AAAGAGG24450.031.874537120
TGTAGAA1551.8476021E-730.941233120
GCCGTGG1551.8476021E-730.941233120
CAGATGC400.005145950230.00392256-57
TTAGTAG400.005168948329.97657116-117
GAGGATA400.00517028329.974987118-119
AATAGGG1004.2498665E-429.97432120
GGAAAGG18250.028.578257120
GTAGGGG8850.028.450203120
TAGATCC6800.028.211124120
TCGTGTG11950.026.086437120
Produced by FastQC (version 0.11.5)