FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33255507
Sequences flagged as poor quality0
Sequence length126
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCT1450.068.245031
CCGATCT1700.056.438919
CTTCCGA1750.051.4010436
TCCGATC1800.049.9719548
CGCTCTT1950.049.209522
CGACCTC904.8789316E-639.991276120
GCTCTTC2700.039.9798473
CGACACC700.002680815534.278233120
GACACTC902.2825466E-433.32606120
CCAACGA800.00515479929.993456120
AACGACC1004.234254E-429.993454120
CTACAGC1203.4989556E-529.993454120
GCAATAC1004.234254E-429.993454120
CACCGAA850.006931862328.229134120
ACGCCAA1504.9746814E-627.993889120
ACAAGCC1954.4281478E-827.686264120
ACAACCC4350.027.580185120
GATAACC1755.316124E-727.422586120
CATACAA2204.7366484E-927.266779120
ACCTAGA900.009161426.660849120
Produced by FastQC (version 0.11.5)