FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28426948
Sequences flagged as poor quality0
Sequence length126
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCT1850.090.992321
CCGATCT1950.089.203429
CTTCCGA2150.083.695276
TCCGATC2100.082.831758
CGCTCTT2050.081.96432
GCTCTTC2150.080.943153
TTCCGAT2950.063.0315177
TCTTCCG3850.046.760715
CTCTTCC4050.042.9698224
CTCACAC1503.3069227E-936.135212120
GATCCAC350.002675569634.291973106-107
CGGGTCC350.002678410434.2845566-67
CGATCTA1004.7293724E-1132.99028810-11
CTCTACC1152.5464695E-531.421923120
CTATCAT800.005055763330.112677120
ACTACTA1602.3441316E-730.112677120
ACCCGAC800.005055763330.112677120
CCTATAT800.005055763330.112677120
CCAATGT400.005143278730.00722112-113
GCAACTC400.0051489930.000408118-119
Produced by FastQC (version 0.11.5)