FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32625112
Sequences flagged as poor quality0
Sequence length126
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA779210.23883749425902354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTCC2500.033.740517120
CCGCAGG750.003679058732.133827120
GTTGACT400.005150930529.99810416-17
TCTCGGG13900.028.174892120
TAGAAGT1556.2038016E-627.210094120
CACTCGG900.0089673226.778189120
AGGCCGG900.0089673226.778189120
GATATGG900.0089673226.778189120
GTAGATT12400.026.724201120
ACGGCTG709.996537E-525.709475116-117
TCGCCGG6500.024.100368120
CATAAGG1250.001519396324.100368120
CATGTGA751.5987737E-423.991375118-119
ACGGTAG1551.9362856E-423.322939120
AGGAGTT1300.001908946823.17343120
TGTTGAG1300.001908946823.17343120
ACAGCGG1300.001908946823.17343120
GCTGAGA802.475572E-422.491913118-119
GAATCGG1350.002377166222.315157120
GAAATGG2203.7522004E-621.909426120
Produced by FastQC (version 0.11.5)