FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20230227
Sequences flagged as poor quality0
Sequence length126
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCT208.1898423E-490.045231
CTTCCGA250.001991028471.992216
TCCGATC401.7020007E-459.991738
TCTTCCG350.007547437751.4232645
TATGCCC708.643765E-751.42161120
CCAGTCT1800.039.994587120
TATACCA400.005151916329.9969108-109
GTACACA800.005152665529.99594120
ACCCTTC400.005152725629.99594116-117
CACCAAA800.005152665529.99594120
CACTGAA1254.6198496E-528.796103120
ACCCGTT850.006928994328.231472120
ACATCTC557.3916017E-427.269037116-117
TTATACC450.00915777826.663023114-115
TTCCGAT900.00915772726.6629927
ACGTCTT22250.026.423391120
CCATCTT808.209387E-626.246382112-113
GCATACT1858.6292494E-725.942432120
ACTTCAT2851.05501385E-1025.259739120
AACTCCC3201.6370905E-1124.3717120
Produced by FastQC (version 0.11.5)