FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20230227
Sequences flagged as poor quality0
Sequence length126
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA381740.1886978331978183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG2850.044.22701120
GTAGATT3750.035.213123120
GAGTCGG1254.6038727E-528.810738120
TCGCCGG1055.614513E-428.582079120
GCGTCGG20850.026.196814120
TCGTGTT5450.024.229214120
GTCTAGG1056.6850254E-520.008867114-115
CCCGGTG750.004474753520.008743112-113
TGAGGGG1056.685661E-520.00862110-111
GCTGTGG750.004474913720.00862110-111
GAGTGTT1907.8528974E-418.954433120
AGTGTAA7450.018.530396120
TCGTTTG850.009216309517.654882114-115
TGGAGAT850.009216309517.654882114-115
GTTGGTC850.00921696617.654663110-111
TAGAGCT1050.00153642417.15035112-113
AGAGCGC22150.017.071825120
GTGTACG1252.542597E-416.807343112-113
GCGCCGG3255.9839385E-616.621578120
CGGTGGG3255.9839385E-616.621578120
Produced by FastQC (version 0.11.5)