FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20259852
Sequences flagged as poor quality0
Sequence length126
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTT7500.036.79771120
CTCATAA851.6299279E-435.291924120
GCTACAA21450.031.326723120
GGCTACT2451.6370905E-1129.385927120
CATAAGC450.00915445626.66501118-119
ATGCCGG2256.008122E-926.665009120
TATGCCC4200.024.284204120
CGCCTGA2052.1083597E-623.413177120
CTACATT8800.023.180376120
CCAGTCC7400.022.701292120
CAGCTAA1350.002436341522.220839120
ACGCTAC700.003008996621.42724118-119
ACTCATA1004.6098412E-520.998695118-119
ACGGCTT4102.1827873E-1120.48653120
CCAGTCT5650.020.175735120
TCATCTA1500.004487625319.998756120
CAATCAA2408.266821E-619.998756120
CATAAAC2459.878404E-619.590618120
AATCCAA2209.593929E-519.089722120
CACTAGT800.006516597218.748835118-119
Produced by FastQC (version 0.11.5)