FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20259852
Sequences flagged as poor quality0
Sequence length126
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA554230.27356073479707554No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATT9050.035.81066120
TCGGTGC1453.6379788E-1226.901571106-107
AGTCTAG2300.026.094595108-109
TCTCGGG6900.025.224123120
AGTGTAA16350.024.593718120
GCTCGGT2200.024.553915112-113
CGTAGCA1101.4176112E-724.552643108-109
GAGTCTA2450.024.496967108-109
CGGTCGC1850.024.332708112-113
GGCGGTC1803.6379788E-1223.341232110-111
GTCTAGG2000.022.507618110-111
GTGCTCG1604.5110937E-1022.506588108-109
TAGAGCT2550.021.18267108-109
TGTAGGC3150.020.956291106-107
CAGTGTA2306.933624E-720.875135120
GTAGCAT1307.0930764E-720.775314108-109
CTAGGGA2253.6379788E-1220.006895112-113
CTGGTCG2103.092282E-1120.006893112-113
CGCTGGT1952.4556357E-1020.006771110-111
GGTGCTC1952.4556357E-1020.005857108-109
Produced by FastQC (version 0.11.5)