FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39415025
Sequences flagged as poor quality0
Sequence length126
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC3050.051.1435478
CCGATCT3400.047.643229
ACGCTCT4000.043.5606581
CGCTCTT4750.036.6412242
CTTCCGA4300.034.8824166
TTCCGAT5600.032.140357
TCTTCCG6450.025.1168695
TGCCGTT9350.021.178661120
CCTTATT27400.020.14807120
AGAGCAA89300.015.791107120
GTCACGG6102.3646862E-1115.739333120
ACGTCTT38300.015.667417120
GGCCTTT21500.015.629524120
AGTCACT26900.014.945784120
TTTTCCC28950.014.3006913
GCCTTAA32650.013.967753120
GCTCTTC11700.013.8463213
TTTTTCC62500.013.251492
TCCAGTA26450.012.931394120
CACGTCG6552.3184293E-711.90963120
Produced by FastQC (version 0.11.5)