FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36412589
Sequences flagged as poor quality0
Sequence length126
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT3250.0108.896099
ACGCTCT3050.0108.413761
CTTCCGA3200.0106.848516
TCCGATC3350.0105.6454548
GCTCTTC3300.0100.034563
CGCTCTT3350.098.5415042
TCTTCCG3700.092.465135
TTCCGAT4350.081.359147
CTCTTCC4100.077.587784
CGATCTG1200.044.98915510-11
TTACCCC1104.3947875E-738.1950154
TTTCCCC6000.036.012443
TTCCCCC4750.035.3806464
TTTACCC1658.6711225E-932.7385833
ATGCCGC750.003720634232.060177120
CGATCTA1450.031.02700410-11
ATCTGCC400.00514918630.00018774-75
TTTTCCC7900.029.6304913
TTCCCTC2251.8553692E-1029.3437145
TACCCCC1254.604567E-528.810195
Produced by FastQC (version 0.11.5)