FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195635151
Sequences flagged as poor quality0
Sequence length126
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCT8900.062.066571
TCCGATC8500.061.387048
CTTCCGA8900.060.6520846
CCGATCT8900.058.6283729
CGCTCTT12450.044.81212
GACCAAA26550.040.73022120
GCTCTTC14900.037.8424763
TCTTCCG15850.034.438655
TTTCCCC78150.027.0946583
TTCCCCC72450.026.5771164
TTCCGAT21100.026.434737
GTCACTT17100.022.484987120
ATGCCGC26400.022.30134120
TATGCCC17350.021.468466120
CCAGTCC55150.019.60811120
CACGAGG38200.019.02965120
TTTTCCC115550.018.6382962
CTCTTCC31800.017.3540924
GACCAAC53950.016.36947120
CCAATCC9900.015.170979120
Produced by FastQC (version 0.11.5)