FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195635151
Sequences flagged as poor quality0
Sequence length126
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2570820.13140890002942263No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2376020.12145158923919556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG48200.029.513699120
GTCGTGG62950.028.700737120
TCGCCGG28300.027.784792120
GCGTCGG243950.026.425655120
CGTGGTC11900.024.96461340-41
TCGTGTG127000.022.827885120
CCGTGGT13300.022.33753838-39
TCCGTGG12350.022.11190638-39
CGGTGGG45950.021.031132120
GTAGATT30600.020.988659120
AAAGAGG279050.020.520533120
AGAGCGG175850.020.480057120
AGTGTAA49150.020.272478120
CGGAAGG150500.019.542578120
GGAAAGG184800.018.871069120
GTAGGGG83200.018.757385120
TCGTGTT40450.018.103544120
TGGTCCC13050.017.24640342-43
TAGCGTG3154.4438657E-617.14961120
GATTAGG2502.5434984E-416.806616120
Produced by FastQC (version 0.11.5)