FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57048408
Sequences flagged as poor quality0
Sequence length126
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCAAA212450.012.417239120
CTTGAAA2382950.07.1351585120
GCTTGAA2526250.06.88539120
TGCTTGA2666650.06.708078120
CAGATCG575300.06.5118394120
CTGCTTG2838350.06.3468485120
CACGTCG61500.06.169829120
TTCTGCT3254900.06.1526423120
TTATCGC148950.06.020891724-25
GTCGAAC44050.06.016092120
CGTCTTC3703500.05.9765797120
CCGTCTT3886600.05.9708595120
CTTCTGC3460200.05.9564366120
TCTGCTT3071650.05.9373384120
ATGCCGC51200.05.881766120
AAAAAAC1050300.05.8126736118-119
AAAAAAA20622600.05.8025436120
TCTTCTG3642800.05.775252120
GCCGTCT4062100.05.6965833120
GTCTTCT3677200.05.690105120
Produced by FastQC (version 0.11.5)