FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19180704
Sequences flagged as poor quality0
Sequence length126
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA299550.15617257844133353No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCTG152.6146427E-4119.9908120
GTAGATT3150.028.569237120
GTTGACG450.009149685526.667854118-119
GGGTGCT450.009149685526.667854118-119
AGCGTCC2352.6896487E-722.976961120
TTGTTCG1703.7223916E-421.174847120
GAGATGG1500.004487991319.998466120
GTCGTGG5800.019.653666120
AGTGTAA7450.019.327377120
TCGTGTT3854.5401976E-918.699863120
GCGTCGG16900.018.460123120
ACGGCAG1000.001109859418.00303334-35
TCTCGGG2452.1876389E-417.141544120
GCGCCGG2904.213398E-516.550455120
TCGTGTG12700.016.061762120
AAAGAGG21200.015.281848120
CGGAAGG11200.014.99885120
CCAGGAC1250.00492904114.39934638-39
CCGGGTA1300.0063863213.847692114-115
ATCGCGG1300.006401391213.84276588-89
Produced by FastQC (version 0.11.5)