FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20298653
Sequences flagged as poor quality0
Sequence length126
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTCC453.0449295E-453.32931120
ACGACAC505.120994E-447.99638120
TAGCCCG300.001256728239.997086116-117
CTGCTCT600.001256728539.996986120
CATCCAA600.001256728539.996986120
TACATCC1401.7480488E-938.568523120
CAACCTA957.0647657E-637.89188120
ACCTACC801.1410986E-437.497173120
CTTTCAG650.001862273836.920299
CTCGTAA1554.7293724E-934.836082120
AACCGAC350.00267894634.28313106-107
TCTCACT700.002678914534.28313120
TCACGGG1251.1991287E-633.59747120
ATGCCGC3850.032.724804120
CAACCAA4050.032.590137120
TCACACA1501.3764657E-731.99759120
CAGCTAA953.1321487E-431.576567120
TCATACT953.1321487E-431.576567120
CTGCTTT6850.030.654623120
CTCAATC400.00515109129.997887114-115
Produced by FastQC (version 0.11.5)