FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20298653
Sequences flagged as poor quality0
Sequence length126
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA465520.22933541452233308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTCC1800.053.35601120
GTAGATT8700.030.357733120
GTGAGGT850.006910058728.247301120
TTGGCGC450.00913547526.676363106-107
TCGCCGG3500.025.72522120
AGTGTAA22300.025.57141120
TCTCGGG7650.025.108711120
ACTCGGG2201.4157376E-724.555893120
ATCAGTG1703.7098088E-421.185476120
TGCACGC853.7232554E-421.17420454-55
CGCCGTT7700.018.709251120
TCGTGTT10100.017.235048120
CGGTGGG6800.016.771835120
TGGCCGT2500.00491356514.406123120
CGTTGGT2600.00637291113.852042120
GGTATCA1550.001300263613.555301118-119
GGTGGTT4651.2186863E-512.908713120
CGGTAGT2251.2821588E-412.006124118-119
AGTCGCC3202.252229E-611.256129114-115
GTGGACG4852.5744256E-411.138755120
Produced by FastQC (version 0.11.5)