FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62189523
Sequences flagged as poor quality0
Sequence length126
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCT3250.077.5371861
CTTCCGA3700.076.20436
CCGATCT3950.072.897949
TCCGATC4000.071.986778
GCTCTTC4750.059.3548433
CGCTCTT4300.058.599542
TCCGACA350.007541228651.434097120
CTCTTCC6300.044.751314
ACGTCCC558.1832166E-443.64105120
TTCCGAT7200.041.659087
TCTTCCG6800.041.46045
ACGAACC700.002676557334.2894120
CTAGTCC902.2782097E-433.33691120
AGTCACC15600.030.772535120
CAAATCC3150.030.479465120
ATGCCGC15550.027.012548120
TATGCCC14450.026.161978120
CTGCTTT19400.024.1263120
CAACGCA1250.001555811124.002577120
TCAGTCC3153.7107384E-1022.859598120
Produced by FastQC (version 0.11.5)