FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52446528
Sequences flagged as poor quality0
Sequence length126
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1619310.308754470839328No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790740.15077070497402612No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGG600.001256287839.9999581
TCTCGGG5350.032.53883120
GTAGATT8300.031.099117120
GGGTTAC400.00514921230.00016648-49
GCGTCGG31900.026.344812120
TCGTGTG16100.026.099363120
GTCGTGG10300.024.477654120
GAGTGTG2852.8958311E-923.168907120
TCGCCGG2954.314643E-922.38352120
AGCGGAG1703.7086566E-421.18654120
TCGTGTT8550.021.062643120
AAAGAGG43450.020.58516120
TTGCATG750.004483125620.00231752-53
AGTGTAA10900.019.825937120
AGAGCGG27150.019.677862120
GTAGGGG14400.019.592646120
GAATGGG1600.006496430418.758915120
CGAGAGG1600.006496430418.758915120
AGGAGAT2000.00110758318.008558120
CGGTTGT1700.00921463417.65545120
Produced by FastQC (version 0.11.5)