FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21123099
Sequences flagged as poor quality0
Sequence length126
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCT1100.092.736221
CGCTCTT1100.092.727652
TCCGATC1250.081.5997548
CCGATCT1300.078.46139
CTTCCGA1350.075.5560466
TCTTCCG1500.067.9891745
TTCCGAT1700.059.999967
GCTCTTC1850.055.1268773
CTCTTCC1900.053.675794
CCGAGGA300.001255653440.00404748-49
AACTCCT1352.1842625E-631.113668120
AAAGCCT400.005141827730.00893230-31
AACCCCT450.009148071526.66882746-47
ACGACTG750.00448395520.001644118-119
CGATCTA750.004486167319.9999410-11
TATACTC750.00448862619.998045114-115
AGTCACA1550.00542012519.356432120
ACGTCTT10500.018.858694120
TACCTCG800.006511169518.751541118-119
ACACGAC800.006518915318.747679116-117
Produced by FastQC (version 0.11.5)