FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43368241
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC4850.0102.6534358
CTTCCGA5100.099.971186
CGCTCTT5500.094.0201952
GCTCTTC5800.090.1937943
TTCCGAT7400.069.709637
CTCTTCC7650.066.0232244
ACCCGTG150.00409976259.999110-111
TCTTCCG11100.046.0439725
ACGCTCT11700.044.2443121
CCGATCT12800.038.8960239
CAGCCAA750.00369640332.103120
TACAGCA750.00369640332.103120
CTCAGTA953.0723683E-431.680592120
ACGCCAT800.005069043530.096563120
CATGCAA800.005069043530.096563120
CTACCAA1203.41797E-530.096563120
TTCACCA900.00900941526.752499120
CCTACAG600.001227918424.99781118-119
ACAGCAA3201.6370905E-1124.453457120
CCTACAT1250.001527918624.077251120
Produced by FastQC (version 0.11.5)