FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43368241
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA579050.13351936501182973No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN471830.10879620411627948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAGG1004.1553672E-430.08993120
ATGCTGG800.005074509430.08993120
TATGGCC900.00901910626.746603120
GTGGTCC6900.025.292984120
AGTCAGG1250.001529881324.071943120
GATGTGA2254.6061596E-621.397282120
AGCATGC1450.003623139620.751673120
GATTAGT1457.901076E-420.751673120
CTGGTAA1754.4341476E-420.633095120
CGCGGTT2106.555219E-520.059952120
TGGACTG1500.004409054320.059952120
CAGCAGG2157.855E-519.593441120
GAGCATT2157.855E-519.593441120
ATGAGTT1856.4484926E-419.517792120
ACAGCTG1702.2311724E-819.4065118-119
AGGTATG2209.3718576E-519.148136120
GCAACGG3202.4244218E-718.806206120
CGAGCTT1600.00640267118.806206120
GCGGATG1600.00640267118.806206120
GTAGTCC1600.00640267118.806206120
Produced by FastQC (version 0.11.5)