FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43158732
Sequences flagged as poor quality0
Sequence length126
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA2150.092.068196
TCTTCCG2200.090.226715
TCCGATC2300.083.455758
GCTCTTC2550.080.2015153
CGCTCTT2400.080.201332
ACGCTCT3550.055.9739881
CTCTTCC4050.050.4972534
TTCCGAT4850.040.8137367
CAGTCAA37300.036.791496120
ACGACTA1504.8458896E-628.08848120
TCACGAA126450.027.560123120
GTCACAA3500.027.515245120
CACGATT7350.027.023901120
TTTCCCC3007.2759576E-1226.0654953
TTTACCC1409.814488E-525.779063
CTGCTTA5100.023.603764120
TATGCCC3603.6379788E-1223.407064120
TTTTCCC5200.023.1350543
ATGCCGC3505.4569682E-1122.356136120
TTCCTCC2203.8065737E-621.873144
Produced by FastQC (version 0.11.5)