FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57746480
Sequences flagged as poor quality0
Sequence length126
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460030.25283445848127883No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1454370.2518543121589402Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG50250.027.09117120
TCGCCGG33550.021.986217120
GTAGATT37050.018.776201120
CGCCGTT27300.018.672146120
AGTGTAA77450.018.118923120
CGGTGGG52050.014.863014120
AGTCGTT2856.9371646E-414.729606120
GTACGGG2450.004322977314.686663120
GTGCGAG3702.061942E-514.587427120
GTGAGCG5653.3916876E-813.798531120
CGCGGTT3502.1004898E-413.707552120
TCACGGG3552.3687689E-413.514487120
ATGCGAG3753.7652077E-412.7937155120
TATCGGG7557.039489E-1012.708989120
TGGCGCC3350.002348485212.531157120
AAAGAGG269100.012.123364120
TGCGCCA5606.326749E-611.7860934
TTGCGCC3650.004447860611.5063113
ACAGCGG7851.7014827E-811.459339120
TAGCGGG3700.004939315411.345777120
Produced by FastQC (version 0.11.5)