FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18146310
Sequences flagged as poor quality0
Sequence length126
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC509320.2806741425667257TruSeq Adapter, Index 8 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT454.0652594E-879.995439
CTTCCGA602.9788316E-759.9988866
CGCTCTT859.113137E-1056.4837072
TCCGATC655.1800635E-755.381458
GCATCAT453.0412513E-453.34234120
ACGCTCT1003.8398866E-948.0231931
TCCTCTA1003.8508006E-948.008102120
CGCCAGT1800.046.67455120
GCTCTTC909.114592E-846.6667023
GCATGCC802.1647666E-645.0076120
CTGCATA558.180588E-443.64373120
ACCCTAC300.00125654439.998264116-117
TGTGCCG1759.094947E-1237.720654120
TGCTGCT1759.094947E-1237.720654120
CTACGAA650.00186003736.92931120
GCCACAA700.002675701234.291504120
CAGCAAT350.002678702434.28375686-87
ATCAACC1606.4319465E-933.7557120
TGTACCG750.003750732.007432
CATACTC1152.6094787E-531.309635120
Produced by FastQC (version 0.11.5)