FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18146310
Sequences flagged as poor quality0
Sequence length126
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG568440.3132537689480671Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA524790.2891992917568365No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGGGC350.00753261751.4488147
GTGGGCC1055.6287635E-428.56968120
TATGCGC900.009134009526.6771627
TCTCGGG10950.026.299757120
GTAGATT10800.024.442947120
GTGTCAT1301.6513679E-518.4573890-91
ATGACCG3654.9900336E-818.0900084
GTCTAGG2007.3068804E-917.99594790-91
AGTGTAA22500.017.865572120
GTGGTCC1700.00924293317.645977120
ACGCTTG850.00925102917.6433558-59
ATCCGGG1100.002102661216.364393118-119
TCGCCGG6301.8189894E-1216.189484120
CTAGGGA2455.169568E-915.914803592-93
TGACCGA4202.4716792E-715.7210785
GGTAGCA1150.00282882615.652897118-119
CTCGGCG2751.4442776E-915.27044586-87
TGTAGGC2952.382876E-1015.25195786-87
CGGTCGC2204.2207648E-714.99664392-93
AGGTCTC1406.053503E-414.99662290-91
Produced by FastQC (version 0.11.5)